Reconstitution & Filtration Station
TOPICS WE WILL COVER
Basic Beginners Reconstitution Guideline
- SUPPLIES : What you need to start your reconstitution journey
- RECONSTITUTION : How to properly reconstitute research peptides
- FILTERING : Filtering techniques and best practices
*This is for research purposes only*
Supplies
Essentials for Reconstitution:
- Lyophilized research peptide vial (your peptide in powder form)
- Bacteriostatic water (HOSPIRA is preferred)
- Insulin syringes (0.5 mL or 1 mL) – for drawing and dosing
- Alcohol prep pads – to sterilize vial tops and surfaces
- Sterile mixing syringe (1–3 mL) – to draw and add diluent to vial
- Mixing needle (18G–22G) – larger gauge helps draw thick solutions
- Sharps container – safe disposal of used needles/syringes
Essentials for Filtering (if applicable):
- 0.22 micron sterile syringe filter – for removing particles or bacteria
- Sterile empty vial – to transfer filtered solution into
- Additional sterile syringe (3–10 mL) – to push liquid through the filter
- Extra mixing needles – for transferring without dulling tips and venting
Optional but Helpful Supplies:
- Gloves – for extra cleanliness
- Labeling stickers or tape – to mark concentration, date, compound name
- pH test strips – if you’re adjusting pH
Reconstitution
Reconstitution is the process of mixing a freeze-dried (lyophilized) peptide powder with bacteriostatic water (Hospira is preferred)— to turn it into a usable liquid form.
Basic Steps to Reconstitute a Peptide (without filtering):
First you must decided how much BAC water you want to use. here is a great calculator to use.
1. Gather Your Supplies
2. Sanitize Everything:
Wipe the tops of both vials (peptide and BAC water) with alcohol swabs.
Wash your hands or wear sterile gloves if you have them.
3. Draw Up the Liquid:
Using your syringe, draw up the exact amount of BAC water you plan to use.
4. Slowly Add the Liquid to the Research Peptide:
Insert the needle into the peptide vial and let the liquid drip gently down the side of the vial — do not spray forcefully onto the puck!
5. Let It Fully Dissolve:
Swirl the vial gently if needed (never shake hard).
Some peptides dissolve almost instantly; others may take a few minutes.
6. Label Your Vial:
Mark the vial clearly with the peptide name, concentration, and date of reconstitution.
7. Store Properly:
Store your reconstituted peptide in the refrigerator (between 36°F–46°F / 2°C–8°C).
Filtering
Filtering is the process of passing a reconstituted peptide solution through a sterile micron filter (usually 0.22 microns) to help remove tiny particles, potential contaminants, and some bacteria.
This step is done after reconstitution (mixing the peptide with a diluent like bacteriostatic water) to create a cleaner, clearer, and safer solution for research purposes.
Basic Steps To Filter a peptide
1. Gather supplies
2. Sanitize Everything
Wash your hands or wear gloves. Wipe the tops of all vials with alcohol prep pads.
3. Draw Up the Reconstituted Peptide (see above)
Use a sterile syringe to draw up the full volume of your reconstituted peptide.
4. Attach the Sterile Filter
Remove the drawing needle and attach a 0.22 micron syringe filter to the syringe tip.
5. Attach a New Sterile Needle
If your sterile vial has a rubber stopper, attach a new sterile needle to the other side of the filter for piercing the vial.
6. Insert a sterile needle into the sterile vial to act as a vent.
This equalizes pressure and prevents spray-back or backflow when filtering.
7. Insert Into Sterile Vial
Insert the needle/filter combo into the new sterile vial.
8. Slowly Push the Solution Through the Filter
Press the plunger gently and evenly — don’t force it. The liquid will pass through the filter into the sterile vial. This removes particles, debris, and potential contaminants.
9. Remove Both Needles
Once filtering is complete, remove both the filter needle and the vent needle.
10. Label Your New Vial
Mark it with the peptide name, concentration, and date.
11. Refrigerate
Store your filtered peptide in the refrigerator (usually between 36°F–46°F / 2°C–8°C).
Disclaimer: All information on this site is intended for research purposes only.
Disclaimer: All information on this site is intended for research purposes only.
Disclaimer: All information on this site is intended for research purposes only.
Disclaimer: All information on this site is intended for research purposes only.
Disclaimer: All information on this site is intended for research purposes only.
Disclaimer: All information on this site is intended for research purposes only.
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